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- Bioss
- bsm-43011M
- 2025年10月16日
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- 规格：
50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-43011M |
| 英文名称 | RBP4 Mouse mAb |
| 中文名称 | 视黄醇结合蛋白4单克隆抗体 |
| 英文别名 | plasma retinol binding protein 4; Plasma retinol-binding proteinv; Plasma retinol-binding protein(1-176); prbp; PRO2222; RBP; RET4_HUMAN; Retinol binding protein 4; retinol binding protein 4 interstitial; Retinol binding protein 4 plasma. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human RBP4 protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 21 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M PBS (pH7.4). |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Blood > Serum Proteins Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Signal Transduction > Metabolism > Vitamins / Minerals |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the calycin superfamily. Lipocalin family. |
| 功能 | Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Retinol binding protein 4, also known as RBP4, is a transporter protein for retinol (vitamin A alcohol). RBP4 has a molecular weight of approximately 21 kDa and is encoded by the RBP4 gene in humans. It is mainly, though not exclusively, synthesized in the liver and circulates in the bloodstream bound to retinol in a complex with transthyretin. RBP4 has been a drug target for ophthalmology research due to its role in vision. RBP4 may also be involved in metabolic diseases as suggested by recent studies. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:400-800} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBP4) Monoclonal Antibody, Unconjugated (bsm-43011M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBP4) Monoclonal Antibody, Unconjugated (bsm-43011M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human renal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBP4) Monoclonal Antibody, Unconjugated (bsm-43011M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Sample:
Lane 1: Recombinant human RBP4 protein, His (HEK293)
Primary: Anti-RBP4 (bsm-43011M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Lane 1: Recombinant human RBP4 protein, His (HEK293)
Primary: Anti-RBP4 (bsm-43011M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
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RBP4 Mouse mAb(bsm-43011M)-50ul/100ul/200ul/200ug
￥1180 - 5600
