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| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-23751R |
| 英文名称 | IDO1 Rabbit pAb |
| 中文名称 | 吲哚胺2,3-双加氧酶抗体 |
| 英文别名 | Indoleamine 2, 3-dioxygenase; 3-dioxygenase; I23O1_HUMAN; IDO 1; IDO; IDO-1; INDO; indolamine 2, 3 dioxygenase; Indole 2 3 dioxygenase; indoleamine 2 3 dioxygenase 1; indoleamine 2 3 dioxygenase; Indoleamine 2, 3-dioxygenase 1; Indoleamine pyrrole 2 3 dioxygenase; Indoleamine-pyrrole 2; indoleamine-pyrrole 2, 3 dioxygenase. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse IDO1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Lipoprotein metabolism Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Signal Transduction > Metabolism > Amino Acids |
| 相似性 | Belongs to the indoleamine 2,3-dioxygenase family. |
| 功能 | Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Lymph node tissue lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human A431 cell lysates
Primary: Anti-IDO (bs-23751R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD

Lane 1: Mouse Epididymis tissue lysates
Lane 2: Human Hela cell lysates
Lane 3: Human Molt-4 cell lysates
Primary: Anti-IDO (bs-23751R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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