Blank control（black line）:HepG2.
_x000D_
Primary Antibody (green line): Rabbit Anti-Phospho-HSD17B13 (Ser33) antibody (bs-22308R)
_x000D_
Dilution:1ug/Test;
_x000D_
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_
Dilution: 0.5ug/Test.
_x000D_
Isotype control（orange line）: Normal Rabbit IgG
_x000D_
Protocol
_x000D_
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-22308R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-22308R |
| 英文名称 | phospho-HSD17B13 (Ser33) Rabbit pAb |
| 中文名称 | 磷酸化17β羟基类固醇脱氢酶13抗体 |
| 英文别名 | HSD17B13(phospho S33); HSD17B13(phospho Ser33); phospho-HSD17B13(S33); p-HSD17B13(Ser33); 17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; DHB13_HUMAN; HMFN0376; Hsd17b13; SCDR9; Short-chain dehydrogenase/reductase 9; UNQ497/PRO1014. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human HSD17B13 around the phosphorylation site of Ser33 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Signal Transduction > Metabolism > Energy Metabolism |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| 功能 | Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 17 beta-HSD13 (17 beta hydroxysteroid dehydrogenase type 13), also designated Short-chain dehydrogenase/reductase 9 (SCDR9), belongs to the 17 beta-HSD family of proteins, which regulate the availability of steroids within various tissues throughout the body. 17 beta-HSD13 is a 300 amino acid secreted protein that is highly expressed in liver and is also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. The gene encoding 17 beta-HSD13 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
_x000D_ Lane 1: Human U-2 OS cell lysates
_x000D_ Lane 2: Human HL-60 cell lysates
_x000D_ Lane 3: Human SH-SY5Y cell lysates
_x000D_ Lane 4: Human U937 cell lysates
_x000D_ Lane 5: Human HepG2 cell lysates
_x000D_ Primary: Anti-Phospho-HSD17B13 (Ser33) (bs-22308R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 34 kDa
_x000D_ Observed band size: 35 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Human U-2 OS cell lysates
_x000D_ Lane 2: Human HL-60 cell lysates
_x000D_ Lane 3: Human SH-SY5Y cell lysates
_x000D_ Lane 4: Human U937 cell lysates
_x000D_ Lane 5: Human HepG2 cell lysates
_x000D_ Primary: Anti-Phospho-HSD17B13 (Ser33) (bs-22308R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 34 kDa
_x000D_ Observed band size: 35 kDa
_x000D_ _x000D_
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HSD17B13 (p-S33) Rabbit pAb(bs-22308R)-50ul/100ul/200ul
￥1180 - 2800
