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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
store at -20 °C. avoid freeze/thaw cycles
- 保质期:
6-12个月
- 英文名:
Yeast Poly(A) Polymerase
- 库存:
99
- 供应商:
Biorbyt
- 规格:
30000 Units
recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly(A) Polymerase
产品名:Yeast Poly(A) Polymerase
货号:orb1733518
产品中文名:Yeast Poly(A) Polymerase
产品详情链接:https://www.biorbyt.com/yeast-polya-polymerase-orb1733518.html
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文献和实验Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
poly(A) analysis in mouse oocytes (1 ), Drosophila oocytes and embryos (2 –4 ), C. elegans (B. Goodwin, personal communication; M. Gallegos and J. Kimble, personal communication), yeast (J. Engebrecht, personal communication), and cultured cells
Protocol: Fast yeast transformation Add 50 µl carrier DNA to a 1.5 ml tube. scrap cells from plate and add to the carrier DNA. Add in the following order: 1-2 µl DNA-plasmid (up to 30-50 µl) 240 µ
In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
I. General considerations: In situ hybridization of yeast cells is almost identical to mammalian cells, except that the cell wall has to be removed by spheroplasting the cells prior to hybridization. The following protocol
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