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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Avoid freeze/thaw. Store as supplied at -20 to -70°C for 12 months. After reconstitution, store at 2-8°C for 1 month or at -20 to -70°C for 3 months under sterile conditions.
- 保质期:
6个月
- 英文名:
Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
10 ug
纯度:> 95 % by SDS-PAGE and HPLC analyses.
蛋白序列:TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
分子量:Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
生物活性:Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
内毒素:Less than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.
来源:Escherichia coli
形态:Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Uniprot ID:P43029
Note:For research use only.

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文献和实验Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
实验步骤 There are many applications for Basement Membrane Matrix which require different thicknesses and concentrations. In general, a protein concentration > 9 mg/ml, is used for differentiation studies of primary cells
Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors
and macrophages are produced without added growth factors from murine long-term bone marrow cultures (2 ), while lymphoid cells (3 ,4 ) and megakaryocytes (reviewed in ref. 5 ) can be induced under certain conditions.
of differentiating into the three primary phenotypes—neurons, astrocytes, and oligodendrocytes’found in the adult mammalian central nervous system (CNS). A defined serum-free medium supplemented with epidermal growth factor (EGF) is used to maintain the NSCs
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