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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 保质期:
6个月
- 英文名:
Recombinant Escherichia coli CsgBAC operon transcriptional regulatory protein (csgD)
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 90% as determined by SDS-PAGE.
生物来源:Escherichia coli (strain K12)
蛋白序列:MFNEVHSIHGHTLLLITKSSLQATALLQHLKQSLAITGKLHNIQRSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWPHINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR
分子量:29 kDa
表达区域:1-216aa
标签:N-terminal 6xHis-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:E.coli
形态:Liquid or Lyophilized powder
Uniprot ID:P52106
Note:For research use only.

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文献和实验In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7. This technology requires flanking DNA to be cloned into a temperature sensitive
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
expressed open reading frames (ORFs). In Escherichia coli , about 70 members of this group have been identified to date and further estimates assume hundreds of sRNAs per bacterial genome. Regulation of gene expression by sRNAs is predominantly mediated
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