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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Avoid freeze/thaw. Store as supplied at -20 to -70°C for 12 months. After reconstitution, store at 2-8°C for 1 month or at -20 to -70°C for 3 months under sterile conditions.
- 保质期:
6个月
- 英文名:
Recombinant Human Trefoil Factor 1
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
5 ug
产品别名:Breast Cancer Estrogen-inducible Protein, PNR-2, Polypeptide P1.A (hP1.A), Protein pS2
纯度:> 97 % by SDS-PAGE and HPLC analyses.
蛋白序列:EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF
分子量:Approximately 13.2 kDa, a homodimer consisting of two 60 amino acid polypeptide chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
生物活性:Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of �� 100 IU/mg.
内毒素:Less than 1 EU/ug of rHuTFF1 as determined by LAL method.
来源:Escherichia coli
形态:Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Uniprot ID:P04155
Note:For research use only.

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文献和实验BioSpectrum Asia:从米粒生产重组蛋白用于工业和医学
this technology to successfully produce recombinant Human Serum Albumin (OsrHSA), recombinant alpha-1 antitrypsin (OsrAAT), recombinant acidic fibroblast growth factor (OsraFGF) and recombinant basic fibroblast growth factor (OsrbFGF). The expression level ranges
摘要 基因重组蛋白在大肠杆菌中表达时,由于表达量高,往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率,是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述,为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止,人们表达的重组蛋白质已有4000多种,其中用E.coli表达的蛋白质要占90%以上,尽管基因重组技术为大规模生产目标蛋白质提供
相关专题 The Interleukin 1 family is a group of 11 beta trefoil cytokine s. They play a central role in the regulation of immune and inflammatory responses. The history of discovery of these cytokines begins
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