- ￥3120
- biorbyt
- 英国
- orb1905948
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Avoid freeze/thaw. Store as supplied at -20 to -70°C for 12 months. After reconstitution, store at 2-8°C for 1 month or at -20 to -70°C for 3 months under sterile conditions.
- 保质期：
6个月
- 英文名：
Recombinant Human Sonic Hedgehog N-Terminus
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
5 ug
产品别名：HHG-1
纯度：> 98 % by SDS-PAGE and HPLC analyses.
蛋白序列：IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
分子量：Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
生物活性：Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x103 IU/mg.
内毒素：Less than 1 EU/ug of rHuSHH as determined by LAL method.
来源：Escherichia coli
形态：Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Uniprot ID：Q15465
Note：For research use only.
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文献和实验Purifying the Hedgehog Protein and its Variants
The purification of recombinant versions of the N-terminal signaling fragment of Sonic hedgehog (ShhN) from E. coli , Hi-5™ insect cells, yeast, and mammalian cell sources reveals diverse post-translational modifications that affect
Identification of Human Pancreatic Cancer Stem Cells
also demonstrate upregulation of molecules important in developmental signaling pathways, including sonic hedgehog and the polycomb gene family member Bmi-1. Of clinical importance, cancer stem cells in several tumor types have shown resistance to standard
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH
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