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- 详细信息
- 技术资料
- 保存条件:
-80°C
- 保质期:
6个月
- 英文名:
Alpha Synuclein Monomers
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
100 ug
产品描述:Human Recombinant Alpha Synuclein Monomers (Type 1)
产品别名:Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein
验证过的应用:In vitro, In vivo, SDS-PAGE, WB
应用笔记:Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.
反应种属:Human
纯度:>95%
生物来源:Human
蛋白序列:MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
分子量:~14.46 kDa
生物活性:100 µM alpha synuclein protein monomer seeded with 10 uM alpha synuclein PFFs in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
标签:No tag
防腐剂:PBS pH 7.4
来源:Recombinant
浓度:2 mg/ml or 5 mg/ml
Uniprot ID:P37840
Note:For research use only.


产品别名:Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein
验证过的应用:In vitro, In vivo, SDS-PAGE, WB
应用笔记:Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.
反应种属:Human
纯度:>95%
生物来源:Human
蛋白序列:MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
分子量:~14.46 kDa
生物活性:100 µM alpha synuclein protein monomer seeded with 10 uM alpha synuclein PFFs in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
标签:No tag
防腐剂:PBS pH 7.4
来源:Recombinant
浓度:2 mg/ml or 5 mg/ml
Uniprot ID:P37840
Note:For research use only.

Type 1 alpha synuclein pre-formed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of Type 1 alpha synuclein pre-formed fibrils is combined with 100 μM of alpha synuclein monomer, as compared to when 10 nM of Type 1 alpha synuclein pre-formed fibrils is combined with 100 μM of alpha Synuclein monomer. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer. Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 μg).
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Alpha Synuclein Monomers 蛋白,orb1822363,biorbyt
¥8450









