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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 保质期:
6个月
- 英文名:
Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5)
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:Membrane-associated RING finger protein 5;Membrane-associated RING-CH protein V MARCH-V;;RING-type E3 ubiquitin transferase MARCHF5
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 85% as determined by SDS-PAGE.
生物来源:Mus musculus (Mouse)
蛋白序列:MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA
分子量:35.6 kDa
表达区域:1-278aa
标签:N-terminal 8xHis-HiBit-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:in vitro E.coli expression system
形态:Liquid or Lyophilized powder
Uniprot ID:Q3KNM2
Note:For research use only.

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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
结构部分由 E1 和 E2 两个亚结构域组成,胞质内区域称为 E3 结构域 [2]。 图片来源于:Coburger I et al.Analysis of the overall structure of the multi-domain amyloid precursor protein (APP) Aβ是由 APP 编码的蛋白经一系列水解酶酶切产生(学霸自行跳过文字,看图脑补即可)。APP 在跨膜分泌过程中共有 2 条水解途径,即不产生 AD 的非淀粉
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
) , generating a binding site for an E3 ubiquitin ligase, which subsequently targets the β-cateninfor rapid proteasomal degradation (23) . Such efficient suppressionof β-catenin levels ensures that Groucho proteins are free tobind Tcf/lymphoid enhancer factor
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