Human Collagen Type IV protein 蛋白,orb750384,Biorbyt产品图

Human Collagen Type IV protein

蛋白,orb750384,Biorbyt
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  • ¥11232
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  • 英国
  • orb750384
  • 2026年08月06日
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    • 保质期:

      6 months from date of receipt.

    • 英文名:

      Human Collagen Type IV protein

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 规格:

      500 ug

    类别: Proteins

    描述: Human Collagen Type IV protein

    别名: Type IV collagen protein, collagen 4 protein, human collagen protein, Collagen alpha-4 (IV) chain protein

    浓度: 1.0 mg/ml

    形态: Liquid (sterile filtered)

    靶点: COL4A1-COL4A6

    应用: ELISA, IHC, SDS-PAGE, WB

    应用稀释比例: IF: 1:100-1:500, WB: 1:1000

    应用说明: For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

    标记物: Unconjugated

    生物来源: Human

    防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid

    纯度: This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).

    来源: Human

    研究领域: Musculoskeletal & Connective Tissue Research

    UniProt ID: P02462

    保存条件: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    备注: For research use only.

    产品细节图片1
    Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA. **P < 0.01 vs. NG; ##P < 0.01 vs. HG + si-NC. NORAD, non-coding RNA activated by DNA damage; NG, normal glucose; HG, high glucose; HMCs, human mesangial cells; si, small interfering RNA; NC, negative control Col. IV, type IV collagen.

    产品细节图片2
    NORAD knockdown inhibits proliferation, inflammation and fibrosis in HG-induced HMCs by regulating the miR-485/NRF1 axis. (G) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs as determined via ELISA. **P < 0.01 vs. si-NC; #P < 0.05 vs. si-NORAD + inhibitor NC; &P < 0.05 vs. si-NORAD + ov-NC. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; NRF1, nuclear respiratory factor 1; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; si, small interfering RNA; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1.

    产品细节图片3
    NORAD reverses the inhibitory effects of miR-485 on HG-induced HMCs. (F) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs measured via ELISA **P < 0.01 vs. miR-NC; #P < 0.05 vs. miR-485 mimics. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1.

    产品细节图片4
    SDS-PAGE Results of Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5.0 µg). Lane 2: Opal Prestained Molecular Weight Protein. Lane 3: Human Collagen Type IV Non-Reduced (5.0 µg). 4-20% Gel, Coomassie Stained. Observed MW: Type IV α1, α2, α3 bands.

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