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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Recombinant Mouse Single-stranded DNA cytosine deaminase (Aicda)
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:Activation-induced cytidine deaminase (AID) (Cytidine aminohydrolase)
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 85% as determined by SDS-PAGE.
生物来源:Mus musculus (Mouse)
蛋白序列:MDSLLMKQKKFLYHFKNVRWAKGRHETYLCYVVKRRDSATSCSLDFGHLRNKSGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVAEFLRWNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIGIMTFKDYFYCWNTFVENRERTFKAWEGLHENSVRLTRQLRRILLPLYEVDDLRDAFRMLGF
分子量:30.6 kDa
表达区域:1-198aa
标签:N-terminal 10xHis-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:Yeast
形态:Liquid or Lyophilized powder
Uniprot ID:Q9WVE0
Note:For research use only.

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文献和实验Simultaneous Single‐Molecule Mapping of Protein‐DNA Interactions and DNA Methylation by MAPit
by probing with cytosine?modifying DNA methyltransferases followed by bisulfite sequencing. Sequencing individual DNA products after amplification of bisulfite?converted sequences permits assignment of the methylation status of every enzyme target site
北大伊成器团队 Nature Method 发文,揭示单碱基编辑脱靶风险!
的结论。 但两个来自中国的团队并不这么认为。 图片来源:Science图片来源:Science2019 年 3 月,来自中科院神经科学研究所的杨辉团队与来自中科院遗传与发育研究所高彩霞团队在 Science 上「背靠背」分别发表了题为 Cytosine base editorgenerates substantial off-target single nucleotide variants in mouse embryos[3] 与Cytosine, but not adenine,base
三句话读懂一篇 CNS:复旦大学研究表明,种花、种草有助预防多种疾病;打呼噜可能增加老年痴呆的风险
器(Cytosine base editor, CBE)存在一定的安全隐患。 2022 年 11 月 10 日,华东师范大学李大力、刘明耀团队以及北京大学的伊成器课题组联合在 Nature Biotechnology 杂志发表研究论文 Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing。 该研究通过对腺嘌呤脱氨酶 TadA-8e 重新设计改造
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