This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Mouse T4(Thyroxine) ELISA Kit
货号:orb1817324
产品中文名:Mouse T4(Thyroxine) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com/mouse-t4thyroxine-elisa-kit-orb1817324.html