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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 应用:
详询
- 规格:
500 ml
IgM-Reducing Assay Diluent
产品名:IgM-Reducing Assay Diluent
货号:orb1821230
产品中文名:IgM-Reducing Assay Diluent
产品详情链接:https://www.biorbyt.com/igm-reducing-assay-diluent-orb1821230.html
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文献和实验reference method. The intra- and inter-assay coefficients of variation (CVs) are 0.7 and 1.5%, respectively, for serum. The CUPRAC assay proved to be efficient for glutathione and thiol-type antioxidants, for which the FRAP (ferric reducing antioxidant
was named by our research group as ‘cupric reducing antioxidant capacity’, abbreviated as the CUPRAC method. The method is comprised of mixing the antioxidant solution with aqueous copper(II) chloride, alcoholic neocuproine, and ammonium acetate aqueous
for 30 seconds. On the last wash transfer to a new tube, spin down and remove as much liquid as possible. Add 50ul reducing sample buffer, heat 2 min. x 95¡C and load 10ul onto a 10% SDS-PAGE. Execute western according to my protocol
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