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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
15.63-1000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
9.38 pg/mL
- 规格:
48 T
产品别名:HMGB1, HMG1, HMG3, SBP-1, Amphoterin
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P63158
靶点:HMGB-1
Note:For research use only.
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文献和实验High-Mobility Group Box-1 Isoforms as Potential Therapeutic Targets in Sepsis
High-mobility group box-1 (HMGB1) protein was originally described as a nuclear DNA-binding protein that functions as a structural cofactor critical for proper transcriptional regulation and gene expression. Recent studies
Fluorescent Biosensors for the Detection of HMGB1 Release
During necrosis and following some instances of apoptosis (in particular in the absence of a proficient phagocytic system), the nonhistone chromatin component high-mobility group box 1 (HMGB1) is released in the extracellular space. In vivo
Measuring DNAProtein Binding Affinity on a Single Molecule Using Optical Tweezers
in the properties of the DNA as a function of added protein concentration and fitting to binding models, the DNA–protein interaction may be characterized and quantified. As an example, the high mobility group protein HMGB1(box A + B) is observed to stabilize dsDNA







