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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
7.81-500ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
4.69 ng/mL
- 规格:
48 T
产品别名:LDL
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDL. You can calculate the concentration of Mouse LDL in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
Pubmed ID:39745717
靶点:LDL
Note:For research use only.
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文献和实验Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
pellet, and tubes. -Adjust the density of the LDL-Plasma to 1.06 using Potassium Bromide (KBr). If density is not adjusted LDL will not spin down. Calibrate pipette to ensure accuracy, using dH2 O. -Add .075gKBr/mL Plasma. -Check density
Oxidized Low-Density Lipoprotein
Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 25 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Yet, Ox-LDL has neither been defined nor characterized, as its components
Antioxidant Activity of Low-Density Lipoprotein
Oxidation of low-density lipoprotein (LDL) is of great interest for epidemiological and clinical diagnostic reasons, as well as for basic scientific research, because it is thought to precede the development of atherosclerotic lesions







