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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
39.06-2500pg/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
23.44 pg/mL
- 规格:
48 T
产品别名:LCN2, Lipocalin 2, Oncogene 24p3, MSFI
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NGAL. You can calculate the concentration of Mouse NGAL in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P11672
靶点:NGAL
Note:For research use only.
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文献和实验人中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin, NGAL)是Kjeldsen等人于1993年在中性粒细胞的特殊颗粒中首先发现的。以往的研究表明NGAL与炎症、胚胎发育、免疫应答、趋化作用、信号转导以及多种肿瘤的发生与发展等过程密切相关。NGAL在乳腺癌、食管癌、结直肠癌、胃癌、慢性粒细胞性白血病等细胞系中均过表达,并在癌细胞的浸润与不良分化中发挥重要的作用。进一步研究证实,NGAL是存在于中性粒细胞和某些上皮
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