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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.31-20ng/mL
- 检测方法:
Competitive
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.19 ng/mL
- 规格:
48 T
产品别名:CRF, Corticoliberin
应用笔记:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
实验时长:1.5H
UniProt ID:Q8CIT0
靶点:CRH
Note:For research use only.
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文献和实验Mammalian adaptation to stressful stimuli requires activation of the type 1 corticotropin releasing hormone (CRH) receptor (CRH-R1), a 415 aminoacid protein that belongs to the large superfamily of 7 transmembrane domain receptors that relay
Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
., and Chrousos, G.P. 1996. In vivo and in vitro characterization of antalarmin, a nonpeptide corticotropin‐releasing hormone (CRH) receptor antagonist: Suppression of pituitary ACTH release and peripheral inflammation. Endocrinology. 137:5747‐5750
Molecular Analysis of Circulating RNA in Plasma
transcripts of human placental lactogen (hPL) , the β-subunit of human chorionic gonadotropin (βhCG ), and corticotropin-releasing hormone (CRH ) were also quantified in plasma of pregnant women. These circulating placental RNA species have provided the fetal
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