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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
7.81-500pg/mL
- 检测方法:
Competitive
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
4.69 pg/mL
- 规格:
48 T
产品别名:N-Acetyl-5-Methoxytryptamine
应用笔记:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MT. During the reaction, Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
实验时长:1.5H
靶点:MT
Note:For research use only.
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