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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
23.44-1500pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenate, serum
- 灵敏度:
6.5 pg/mL
- 规格:
48 T
产品别名:GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB, TGF-PL
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF15. You can calculate the concentration of Human GDF15 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:Q99988
靶点:GDF15
Note:For research use only.
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文献和实验1 月内连登 4 顶刊,这个炙手可热的明星蛋白 GDF15 到底什么来路?
作用。图片来源:Cell Metabolism这是 GDF15 继 2019 年 12 月先后登上 Nature Metabolism 和 Nature 之后,再次出现在顶级期刊上。下面就随着小编来看看 GDF15 这个热门蛋白的来路以及相关的 4 篇论文吧。GDF15 有啥来路?生长分化因子-15(Growth/differentiation factor 15,GDF15)是转化生长因子-β 超家族(TGFβ super family)的一员。此前 GDF15 被发现在创伤过后的多种器官出现明显
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