Human Hemoglobin (HB) ELISA Ki

产品描述：Human Hemoglobin (HB) ELISA Kit

产品别名：HB

应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HB. You can calculate the concentration of Human HB in the samples by comparing the OD of the samples to the standard curve.

实验时长：3.5H

UniProt ID:P68871, P69905

靶点：HBEGF

Note：For research use only.