- ￥3770
- biorbyt
- 0.31-20ng/mL
- 0.16 ng/mL
- orb1807450
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.31-20ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenate and Cell lysate
- 灵敏度：
0.16 ng/mL
- 规格：
48 T
产品别名：IDO-1, INDO
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDO. You can calculate the concentration of Human IDO in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P14902
靶点：IDO
Note：For research use only.
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文献和实验Indoleamine 2,3-Dioxygenase and Regulatory Function: Tryptophan Starvation and Beyond
Indoleamine 2,3-dioxygenase (IDO) is an ancestral enzyme that, initially confined to the regulation of tryptophan availability in local tissue microenvironments, is now considered to play a wider role that extends to homeostasis
Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells
as well as the secretion and presentation of chemokines. Indoleamine 2,3 dioxygenase (IDO) is an enzyme that metabolises tryptophan and is known to be central in the regulation of immune responses. IDO can be expressed on endothelial cells and can alter T-cell responses
Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5-Triphosphate
-2 can induce apoptosis in tumour cells, further supporting the use of this strategy to combat cancer. This chapter describes the in vitro synthesis of siRNA targeting indoleamine 2,3-dioxygenase (IDO), an enzyme that plays a critical role in hindering immunity
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