- ￥3770
- biorbyt
- 31.25-2000pg/mL
- 13.7 pg/mL
- orb1807373
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
31.25-2000pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
13.7 pg/mL
- 规格：
48 T
产品别名：CDF, DIA, HILDA, MLPLI
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LIF. You can calculate the concentration of Human LIF in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P15018
靶点：LIF
Note：For research use only.
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文献和实验Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
Neural Differentiation of Embryonic Stem Cells
of leukemia inhibitory factor (LIF). ES cells appear to have unlimited proliferative capability, and, remarkably, when returned to the inner cell mass after culture and gene manipulation, resume their development and participate fully in the formation
Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol
inhibitory factor (LIF) which is supplemented with 0.5 μM retinol/vitamin A. The cells are passaged every 3–5 days by trypsinization. The pluripotency of the cells is tested by different undifferentiated ES cell-specific markers.
