- ￥3770
- biorbyt
- 31.25-2000pg/mL
- 13.7 pg/mL
- orb1807352
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
31.25-2000pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Cell culture supernatant, serum
- 灵敏度：
13.7 pg/mL
- 规格：
48 T
产品别名：MIF, GIF, GLIF, Mmacrophage migration inhibitory factor (glycosylation-inhibiting factor), macrophage migration inhibitory factor
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIF. You can calculate the concentration of Human MIF in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P14174
靶点：MIF
Note：For research use only.
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文献和实验转移抑制因子 migration inhibitory factor 缩写MIF。是淋巴激活素（lymphokine）之一；是与抗原反应的淋巴球产生的物质，作用于巨噬细胞，具有抑制其运动的活性。这种物质的实质尚不清楚，但在分子量约为7万的蛋白质分区里有这种活性，它也作用于别种动物的巨噬细胞。现在认为，与位于出现迟发过敏性反应的地方积聚单核白血球或者巨噬细胞有关。认为MIF产生于T细胞的说法是有力的，但也有实验报道，认为B细胞产生MIF。
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
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