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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
31.25-2000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
13.7 pg/mL
- 规格:
48 T
产品别名:NTF4, GLC1, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5, neurotrophin 4
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-4. You can calculate the concentration of Human NT-4 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P34130
靶点:NT4
Note:For research use only.
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文献和实验Visualization and Quantitation of Neurotrophin mRNAs
��k et al. 1991). Recently, a mammalian homologue of NT-4 has been molecularly cloned from rat and human (Ip et al., 1992). The same sequence was also recently isolated by Berkemeier et al. (1991) who named it neurotrophin-5 (NT-5
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
. p75 Neurotrophin R (NTR) : p75 NTR, also named low affinity nerve growth factor (NGF) receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor superfamily (Figure 3).83 It binds to NGF, BDNF, NT-3 and NT-4







