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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
62.5-4000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
25.2 pg/mL
- 规格:
48 T
产品别名:OL
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OxLDL. You can calculate the concentration of Human OxLDL in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P78380
靶点:OxLDL
Note:For research use only.
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文献和实验Oxidized Low-Density Lipoprotein
Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 25 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Yet, Ox-LDL has neither been defined nor characterized, as its components
Antioxidant Activity of Low-Density Lipoprotein
Oxidation of low-density lipoprotein (LDL) is of great interest for epidemiological and clinical diagnostic reasons, as well as for basic scientific research, because it is thought to precede the development of atherosclerotic lesions
Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
This procedure involves use of human plasma, a potentially dangerous source of blood-borne disease. Wear long-cuffed gloves, and eye-protection. Day one: -Human Plasma is purchased from the Red-Cross. It should be 3-4 days old







