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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.78-50pmol/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.114 pmol/mL
- 规格:
48 T
产品别名:Pro-ADM
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ADM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pro-ADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pro-ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Pro-ADM. You can calculate the concentration of Human Pro-ADM in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P35318
靶点:Pro-ADM
Note:For research use only.
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文献和实验and allergy testing. Surface blocking, which is crucial for all reliable immunoassays, is especially challenging in antigen down formats and hence often causes problems and faulty results. Using ELISA as an example, we discuss the reason for these problems
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Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
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