Rat Glucagon (GC) ELISA Kit试剂盒

产品描述：Rat Glucagon (GC) ELISA Kit

产品别名：Carbohydrate-Rich Portion of Platelet Membrane Glycoprotein Ib Alpha Polypeptide

应用笔记：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat GC. During the reaction, Rat GC in the sample or standard competes with a fixed amount of Rat GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.

实验时长：1.5H

UniProt ID:G3V6P5

靶点：GC

Note：For research use only.