- ￥3770
- biorbyt
- 0.16-10ng/mL
- 0.10 ng/mL
- orb1807103
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10ng/mL
- 检测方法：
Competitive
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.10 ng/mL
- 规格：
48 T
产品描述：Human Podocalyxin (PCX) ELISA Kit
产品别名：GCTM-2 antigen, Gp2, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1
应用笔记：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human PCX. During the reaction, Human PCX in the sample or standard competes with a fixed amount of Human PCX on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human PCX. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human PCX in tested samples can be calculated by comparing the OD of the samples to the standard curve.
实验时长：1.5H
UniProt ID:O00592
靶点：PCX
Note：For research use only.
产品别名：GCTM-2 antigen, Gp2, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1
应用笔记：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human PCX. During the reaction, Human PCX in the sample or standard competes with a fixed amount of Human PCX on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human PCX. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human PCX in tested samples can be calculated by comparing the OD of the samples to the standard curve.
实验时长：1.5H
UniProt ID:O00592
靶点：PCX
Note：For research use only.
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结果的对照，因此对照品，特别是阳性对照品的基本组成应尽量与检测标本的组成相一致。以人血清为标本的测定，对照品最好也为人血清，因为正常人血清在各种ELISA模式中可产生不同程度的本底。由于大量正常人血甭较难得到，国外试剂盒中的对照品多以复钙人血浆(recalcified human plasma)为原料，即在血浆中加入钙离子，使其中的纤维蛋白质凝固，除去凝块后所得的液体，其组成与血清相似。阴性对照品须先行检测，确定其中不含待测物质。例如HBsAg检测的阴性对照品中不可含HBsAg，最好抗HBs也是阴性
，在板式ELISA中一般采用2mol/L。3.6 阳性对照品和阴性对照品 阳性对照品(positive control)和阴性对照品(negative control)是检验试验有效性的控制品，同时也作为判断结果的对照，因此对照品，特别是阳性对照品的基本组成应尽量与检测标本的组成相一致。以人血清为标本的测定，对照品最好也为人血清，因为正常人血清在各种ELISA模式中可产生不同程度的本底。由于大量正常人血甭较难得到，国外试剂盒中的对照品多以复钙人血浆(recalcified human plasma
Human Podocalyxin (PCX) ELISA Kit试剂盒，orb1807103，biorbyt
￥3770
