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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
31.25-2000pg/mL
- 检测方法:
Competitive
- 适应物种:
All
- 样本:
Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- 灵敏度:
9.55 pg/mL
- 规格:
48 T
产品别名:Prostaglandin E1; PGE1
应用笔记:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin E1(PGE1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin E1(PGE1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin E1(PGE1) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长:1.5H
靶点:PGE1
Note:For research use only.
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文献和实验人 前列腺素E1(PGE1) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆, 组织及相关液体样本中前列腺素E1(PGE1) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人前列腺素 E1(PGE1) 水平。用纯化的人前列腺素 E1(PGE1) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入前列腺素 E1
Separation of Platelets from Whole Blood
g sodim citrate, 8 g citric acid, 500 ml H2 O2 ). Prostaglandin E1 (PGE1 ). ACD buffer (6.25 g sodium citrate.2 H2 O , 3.1 g citric acid anhidrous, 3.4 g D-glucose in 250 ml H2 O). Hepes-Tyrode buffer pH 7.4 (134 mM sodium chloride
活性物质,其中有的能收缩肺血管,如白三烯(leukotriene,LTs)、血栓素A2(thromboxane A2、TXA2)、前列腺素F2a(prostaglandin F2a,PGF2a)等,有的扩张血管,如前列环素(prostacyclin,PGI2)、前列腺素E1(prostaglandin E1 PGE1)等。在肺血管收缩反应中,缩血管物质生成与释放增加,起介导作用;扩血管物质的生成与释放也可增加,起调节作用。两者力量对比决定肺血管收缩反应的强度。组胺作用于H1受体使肺血管收缩,作用于H2受体则使之扩张
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