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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
15.63-1000ng/mL
- 检测方法:
Competitive
- 适应物种:
All
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
9.38 ng/mL
- 规格:
48 T
产品别名:VA
应用笔记:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin AKit(VA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin AKit(VA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin AKit(VA) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长:1.5H
靶点:VA
Note:For research use only.
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V294.PART II,Chapter 5 细胞粘附实验(英文版)
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