- ￥3510
- biorbyt
- 9.38-600ng/mL
- 2.55 ng/mL
- orb1806558
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
9.38-600ng/mL
- 检测方法：
Competitive
- 适应物种：
All
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
2.55 ng/mL
- 规格：
48 T
产品别名：Riboflavin; Riboflavine
应用笔记：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin B2(VB2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin B2(VB2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin B2(VB2) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长：1.5H
靶点：VB2
Note：For research use only.
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文献和实验Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA)
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