- ￥3510
- biorbyt
- 156.25-10000pg/mL
- 45.2 pg/mL
- orb1806554
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
156.25-10000pg/mL
- 检测方法：
Competitive
- 适应物种：
All
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
45.2 pg/mL
- 规格：
48 T
产品别名：c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate
应用笔记：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Guanosine Monophosphate(cGMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Guanosine Monophosphate(cGMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Guanosine Monophosphate(cGMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长：1.5H
靶点：cGMP
Note：For research use only.
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文献和实验Of the 11 families of mammalian cyclic nucleotide phosphodiesterases (PDEs), 5 contain regulatory domains capable of binding cyclic guanosine 5′-monophosphate (cGMP). The best understood of the GAF-containing PDEs is the family of rod (PDE
Visualization of cGMP with cGi Biosensors
Cyclic guanosine 3′–5′-monophosphate (cGMP) is an important signaling molecule in physiology, pathophysiology, and pharmacological therapy. It has been proposed that the functional outcome of an increase of cGMP in a given cell largely
Detection of cGMP in the Degenerating Retina
Cyclic guanosine 3′-5′-monophosphate (cGMP) plays a key role in the physiological process of light detection in photoreceptor cells of the retina. However, there is also growing evidence that cGMP may be critically involved
