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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
31.25-2000pg/mL
- 检测方法:
Competitive
- 适应物种:
All
- 样本:
serum, plasma and other biological fluids
- 灵敏度:
9.55 pg/mL
- 规格:
48 T
产品别名:8-EpiPGF2-α; 8epi-PGF2a
应用笔记:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve.
实验时长:1.5H
靶点:8-epi-PGF2a
Note:For research use only.
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文献和实验and are subsequently released in the free form following cleavage by phospholipase A2 (3 ). The presence of F2 -IPs in vivo was initially reported by Morrow et al. (1 ). The levels of one of the abundant isomers, 8-epi-PGF2α , was shown to increase by up to 200-fold
Oxford Biomedical Research的氧化应激及慢性炎症生物标志物检测
as an index of oxidative stress in vivo, Free Radic. Biol. Med. 28:505-513 (2000).? 2. 15-异前列烷F2t ELISA分析试剂盒(血清、血浆、尿液和组织),货号EA84,96孔板:检测血清、血浆、尿液和组织中15-异前列烷F2t水平。 注:15-异前列烷F2t(15-Isoprostane F2t)别名:8-表-前列腺素F2α/8-异前列腺素F2α/ 8-异前列烷(8-epi-PGF2α/8-iso-PGF2
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