The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle AGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle AGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle AGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle AGC in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Cattle AGC(Aggrecan core protein) ELISA Kit
货号:orb1736704
产品中文名:Cattle AGC(Aggrecan core protein) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com/cattle-agcaggrecan-core-prote-orb1736704.html