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Dog Progesterone (PG) ELISA Ki

t试剂盒,orb1736694,biorbyt
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  • ¥4290
  • biorbyt已认证
  • 0.78-50 ng/mL
  • 0.32 ng/mL
  • orb1736694
  • 英国
  • 2026年01月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      0.78-50 ng/mL

    • 检测方法

      Competitive

    • 适应物种

      Canine

    • 样本

      serum, plasma and other biological fluids

    • 灵敏度

      0.32 ng/mL

    • 规格

      48 T

    产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog PG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PG in the samples is then determined by comparing the OD of the samples to the standard curve.

    产品别名:P4; Pregn-4-Ene-3,20-Dione

    应用笔记:standard: 50 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog PG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PG in the samples is then determined by comparing the OD of the samples to the standard curve

    实验时长:2.5h

    Note:For research use only.

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