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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
0.16-10 ng/mL
- 应用:
详询
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 规格:
48 t/96 t
| 规格: | 48 t | 产品价格: | ¥3190.0 |
|---|---|---|---|
| 规格: | 96 t | 产品价格: | ¥4301.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPN in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Human SPN(Sialophorin) ELISA Kit
货号:orb1736693
产品中文名:Human SPN(Sialophorin) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com/human-spnsialophorin-elisa-k-orb1736693.html
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有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
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