- ￥2816 - 3784
- Biorbyt
- 0.78-50 ng/mL
- orb1736646
- 英国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
0.78-50 ng/mL
- 应用：
详询
- 适应物种：
Human
- 样本：
serum, plasma, cell lysates and other biological fluids
- 规格：
48 t/96 t
| 规格： | 48 t | 产品价格： | ￥2816.0 |
|---|---|---|---|
| 规格： | 96 t | 产品价格： | ￥3784.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD28. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD28. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD28, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD28 in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名：Human CD28(Cluster Of Differentiation 28) ELISA Kit
货号：orb1736646
产品中文名：Human CD28(Cluster Of Differentiation 28) ELISA Kit 酶联免疫试剂盒
产品详情链接：https://www.biorbyt.com/human-cd28cluster-of-differen-orb1736646.html
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文献和实验厂家 6-3201-002 CD3 Fab-TACS® Agarose column Starter Kit IBA 6-8920-050 CD3/CD28 Streptamer® Kit, mouse IBA
实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds
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