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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
1.25-80 ng/mL
- 应用:
详询
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 规格:
48 t/96 t
| 规格: | 48 t | 产品价格: | ¥3190.0 |
|---|---|---|---|
| 规格: | 96 t | 产品价格: | ¥4301.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit
货号:orb1736643
产品中文名:Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com/human-anti-gm1anti-gangliosid-orb1736643.html
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文献和实验a simple ELISA method to detect human anti-antibody response.
suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
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