- ￥3900
- biorbyt
- 0.16-10 ng/mL
- 0.06 ng/mL
- orb1736546
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.06 ng/mL
- 规格：
48 T
产品别名：gamma H2A.X; γH2AX; Phospho-H2AX-S139
应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human γH2AX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human γH2AX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human γH2AX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human γH2AX in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P16104
Note：For research use only.
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ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
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