- ￥3900
- biorbyt
- 0.32-20 ng/mL
- 0.14 ng/mL
- orb1736538
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates and other biological fluids.
- 灵敏度：
0.14 ng/mL
- 规格：
48 T
产品别名：CREB; active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CREB in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P16220
Note：For research use only.
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文献和实验The ability of prototypical second messenger cyclic AMP (cAMP) to positively control transcription of the somatostatin gene was pivotal to the original identification of the transcription factor cAMP response element-binding protein
Detection of β-Adrenergic Receptors by Radioligand Binding
of cyclic AMP as first demonstrated by Sutherland and his colleagues (1 ). Although β-ARs were initially divided into β1 -AR (norepinephrine ≥epinephrine) and β2 -AR (epinephrine > norepinephrine) subtypes by Lands et al. (2 ), a third subtype, the β3 -AR
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Literature Cited Baichoo, N. and Heyduk, T. 1999. DNA‐induced conformational changes in cyclic AMP receptor protein: Detection and mapping by a protein
