- ￥2816 - 3784
- Biorbyt
- 0.32-20 ng/mL
- orb1736534
- 英国
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
0.32-20 ng/mL
- 应用：
详询
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 规格：
48 t/96 t
| 规格： | 48 t | 产品价格： | ￥2816.0 |
|---|---|---|---|
| 规格： | 96 t | 产品价格： | ￥3784.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名：Human Acetyl-H3(Acetyl Histone H3) ELISA Kit
货号：orb1736534
产品中文名：Human Acetyl-H3(Acetyl Histone H3) ELISA Kit 酶联免疫试剂盒
产品详情链接：https://www.biorbyt.com/human-acetyl-h3acetyl-histone-orb1736534.html
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文献和实验Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
between promoter elements and cellular or viral regulatory factors (5 –9 ). The conventional strategy has been to transfect host cells transiently with a plasmid bearing the sequences of interest linked to a chloramphenicol acetyl transferase (CAT) reporter gene
, A.D., Rossi, J.J. and Morris, K.V. (2005) The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells, RNA, 12(2): 256-262.
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