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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
0.16-10 ng/mL
- 应用:
详询
- 适应物种:
Rat
- 样本:
Tissue homogenates and other biological fluids.
- 规格:
48 t/96 t
| 规格: | 48 t | 产品价格: | ¥2816.0 |
|---|---|---|---|
| 规格: | 96 t | 产品价格: | ¥3784.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIK3C2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIK3C2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIK3C2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIK3C2a in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Rat PIK3C2a(Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide) ELISA Kit
货号:orb1736455
产品中文名:Rat PIK3C2a(Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com/rat-pik3c2aphosphoinositide-3-orb1736455.html
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit(cat NO:EKS-420A)?可否说一下应用的经验?注意事项等?还有,该试剂盒的说明书有几处地方不是很懂,请大家帮忙看一下: 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
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