CF594 Antibody Labeling Kit, 1

Labeling Tubulin and Quantifying Labeling Stoichiometry

. Properties of Fluorescent Dyes Used for Tubulin Labeling Back to protocols I. Solutions & Supplies phosphocellulose-purified tubulin (~50 mg = 2-4 3 ml aliquots of 5-10 mg/ml PC fractions) Dye stock in anhydrous DMSO (20-100 mM

Genomic DNA Labeling Protocol

array hybridizations, combine purified probes (Cy5 and Cy3 labeled probes) in new eppindorf tube. Then add: 30-50 ug human Cot-1 DNA (Gibco/BRL; 1 mg/ml stock; blocks hybridization to repetitive DNAs if present on array). 100 ug yeast tRNA

TUNEL labeling

and concentrations will remain consistent for the labeling. B. Add Total volume (50µl,) of Tube 1 (TdT) + remainder of Tube 2 (450µl). Apply 100µl TUNEL reaction mixture (or 100µl Control Label solution for negative control) to each slide.