Maxi FITC Antibody Labeling Ki

TUNEL labeling

Incubate in humid chamber, 60 min., 37°C. 1x PBS wash, 3 times. Wipe around tissue. Apply 100µl anti-FITC-AP conj. ("converter-AP") on each sample. Incubate in humid chamber, 30', 37°C. 1x PBS wash, 3 times. 100mM Tris

Direct Immunofluorescence Labeling

to 1 million per ml. with culture media or PBS. Place 1 ml. of the cell suspension into each of the 12x75 tubes. You will need a tube for each antibody plus the negative control. If you''re doing simultaneous labeling, use one tube for each combination

Genomic DNA Labeling Protocol

  Developed for microarray-based comparative genomic hybridization. Genomic DNA can be labeled with a simple random-priming protocol based on Gibco/BRL's Bioprime DNA Labeling kit, though nick translation protocols work too. I routinely