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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.391-25ng/ml
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Serum,Plasma,Cell Culture Supernatant,Cell or tissue lysate,Other liquid samples
- 灵敏度:
0.234ng/ml
- 规格:
48 T
产品别名:HDL-C|high density lipoprotein cholesterol
实验时长:4 hours
靶点:HDL-C
保存说明:2 - 8 °C for 12 months
Note:For research use only.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
THE MONOCLONALSingle wash, then triple wash the plates as described previously with special attention to the aspiration step. Maintaining the GIG then GAM sequence, aspirate the standards from low concentration (blank) to high concentration (100 pg). In this way
out at about 1-3x105 cells/ml;at high cell density(>2 x 106 cells/ml),a cytokine-independent subclone may grow out relatively quickly.CAVEAT:by favoring selective growth,suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent






