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- 技术资料
- 抗体名:
LC3 Antibody (APG8A) 抗体
- 抗体英文名:
LC3 Antibody (APG8A)
- 应用范围:
IF, WB
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1933570
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 形态:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 亚型:
Rabbit IgG
- 免疫原:
详询
- 规格:
50 ul/100 ul
| 规格: | 50 ul | 产品价格: | ¥2106.0 |
|---|---|---|---|
| 规格: | 100 ul | 产品价格: | ¥3861.0 |
类别: Antibodies
产品子类型: Primary Antibody
描述: Purified Rabbit Polyclonal Antibody (Pab)
别名: Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
应用稀释比例: IF - 1:200, WB - 1:1000
免疫原: This LC3 antibody is generated from rabbits immunized with full-length recombinant human LC3 (APG8a).
研究领域: Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction
UniProt ID: Q9H492, Q9GZQ8
NCBI 参考序列: NP_852610.1, NP_115903.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
备注: For research use only.


产品子类型: Primary Antibody
描述: Purified Rabbit Polyclonal Antibody (Pab)
别名: Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
应用稀释比例: IF - 1:200, WB - 1:1000
免疫原: This LC3 antibody is generated from rabbits immunized with full-length recombinant human LC3 (APG8a).
研究领域: Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction
UniProt ID: Q9H492, Q9GZQ8
NCBI 参考序列: NP_852610.1, NP_115903.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
备注: For research use only.

Western blot analysis of LC3 (APG8a) (arrow) using purified Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2).

Fluorescent image of U251 cells stained with LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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LC3 Antibody (APG8A) 抗体,orb1933570,Biorbyt
¥2106 - 3861











