- ￥7293
- Biorbyt
- orb1927083
- 英国
- 2025年07月13日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Histone H2A.X (Ser139) Antibody 抗体
- 抗体英文名：
Histone H2A.X (Ser139) Antibody
- 靶点：
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from human.
- 浓度：
详询
- 应用范围：
WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1927083
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
POLYCLONAL
- 保存条件：
详询
- 形态：
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
400 ul
Purified Rabbit Polyclonal Antibody (Pab)
产品名：Histone H2A.X (Ser139) Antibody
货号：orb1927083
产品中文名：Histone H2A.X (Ser139) Antibody 抗体
产品详情链接：https://www.biorbyt.com/histone-h2ax-ser139-antibod-orb1927083.html
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文献和实验Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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