- ￥6201
- Biorbyt
- orb1822436
- 英国
- 2026年08月06日
- FC, ICC, IF, IHC, WB
- Rabbit
- Bovine, Drosophila, Frog, Gallus, Human, Mouse, Rat, Sheep
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- 文献和实验
- 技术资料
- 抗体名：
Erk1/2 Antibody 抗体
- 抗体英文名：
Erk1/2 Antibody
- 靶点：
Erk1/2
- 浓度：
1 mg/ml
- 应用范围：
FC, ICC, IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Bovine, Drosophila, Frog, Gallus, Human, Mouse, Rat, Sheep
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1822436
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ul
描述: Rabbit Anti-Rat Erk1/2 Polyclonal
别名: ERK1, ERK2, ERT1, ERT2, MAP kinase 1, MAP kinase 2, MAPK1, MAPK2, MAPK3, p38, p40, p41, p41mapk, p42 MAPK, p44 ERK1, p44 MAPK, PRKM1, PRKM2, PRKM3
应用稀释比例: WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.
应用说明: A 1:1000 dilution was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
免疫原: A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
分子量: 42kDa (ERK2).
防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
纯化方法: Peptide Affinity Purified
特异性: Detects ~44kda (ERK1) and ~42kDa (ERK2).
研究领域: Cell Biology
UniProt ID: P21708
NCBI 参考序列: NP_059043.1
Entrez 基因 ID: 50689
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
per immunoprecipitation. You can use less (as little as 50) but the higher amount gives you a good, easily detectable amount of activity. 9.) To the lysate, add 1.5 μg of anti-ERK antibody (Santa Cruz, SC154 (Erk2) or SC93 (Erk1)), and incubate, with rocking
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