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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
- 保质期:
1年
- 英文名:
DIG-11-dUTP
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
DIG-11-dUTP,DIG-11-dUTP,DIG-11-dUTP,DIG-11-dUTP
产品货号:NU-803-DIGXL
产品规格:5x25ul(1mM)
DIG-11-dUTP; Nucleotides & Nucleosides;

DIG-11-dUTP; Nucleotides & Nucleosides;

DIG-11-dUTP-DIG-11-dUTPstore at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
点击:DIG-11-dUTP-DIG-11-dUTP查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Jena Bioscience公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验[原理] DNA是通过异羟基洋地黄毒苷(digoxigenin,Dig)配基标记的脱氧尿嘧啶核苷三磷酸(dUTP)随机插入结合而被标记。dUTP通过间臂连结类固醇半抗原异羟基洋地黄毒苷酸基,形成Dig-dUTP,杂交反应后,杂交的靶DNA通过酶联免疫法与一个抗体复合物[抗异羟基洋地黄毒苷配基碱性磷酸酶复合物(Dig)Ap]结合,接着在5-溴-4氯-3-吲哚磷酸盐(X-磷酸盐)和硝基四氮唑蓝(NBT)存在下,由酶催化反应,在杂交部位形成蓝紫色带或颗粒。 [操作] 1.转移 通过打点吸渍
Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for the most part, depends on the sensitivity and resolution required. For maximum sensitivity in filter hybridizations radioactive labels, such as phosphorous 32
3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
In this reaction, the enzyme terminal transferase is used to introduce a short tail of fluorescein nucleotides to the 3′-end of an oligonucleotide (1 ) followed by horseradish peroxidase-catalyzed enhanced chemiluminescent detection
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